Abstract
Cell-membrane-mimicking supported lipid bilayers (SLBs) provide an ultrathin, self-assembled layer that forms on solid supports and can exhibit antifouling, signaling, and transport properties among various possible functions. While recent material innovations have increased the number of practically useful SLB fabrication methods, typical SLB platforms only work in aqueous environments and are prone to fluidity loss and lipid-bilayer collapse upon air exposure, which limits industrial applicability. To address this issue, herein, we developed sucrose–bicelle complex system to fabricate air-stable SLBs that were laterally mobile upon rehydration. SLBs were fabricated from bicelles in the presence of up to 40 wt% sucrose, which was verified by quartz crystal microbalance-dissipation (QCM-D) and fluorescence recovery after photobleaching (FRAP) experiments. The sucrose fraction in the system was an important factor; while 40 wt% sucrose induced lipid aggregation and defects on SLBs after the dehydration–rehydration process, 20 wt% sucrose yielded SLBs that exhibited fully recovered lateral mobility after these processes. Taken together, these findings demonstrate that sucrose–bicelle complex system can facilitate one-step fabrication of air-stable SLBs that can be useful for a wide range of biointerfacial science applications.
Highlights
Supported lipid bilayers (SLBs) are nanoarchitectured, cell membrane-mimetic ultrathin (~ 5 nm) layers which are self-assembled on various types of hydrophilic surfaces [1,2,3,4]
3.1 Formation of supported lipid bilayers (SLBs) Bicelle adsorption is a well-established and versatile method for the formation of SLBs in various environments via the adsorption, fusion, and rupture of phospholipid bicelles onto silica surfaces [29, 36, 58, 59]. We extended this approach to form SLBs from sucrose– bicelles mixtures and performed quartz crystal microbalance-dissipation (QCM-D) experiments to scrutinize the effect of sucrose on bicelles-mediated SLB formation
In step 1, the Δf shifts decreased by approximatel y − 46.8 ± 0.6 Hz to − 660.7 ± 15.2 Hz, and the ΔD shifts increased by approximately 18.2 ± 0.3 × 10−6 to 267.2 ± 8.8 × 10−6, in proportion to the 10–50 wt% sucrose concentrations (Additional file 1: Figure S1B–F)
Summary
Supported lipid bilayers (SLBs) are nanoarchitectured, cell membrane-mimetic ultrathin (~ 5 nm) layers which are self-assembled on various types of hydrophilic surfaces [1,2,3,4]. One of the promising methods is the solvent-assisted lipid bilayers (SALB) method, which generates the SLBs on various solid supports based on the phase transition of phospholipids from inverted micelles and monomers in organic solvents to micelles and lamellar vesicles in aqueous buffer by solvent exchange [29,30,31,32] Another useful method involves the adsorption of bicelles, which have a disk-like nanostructure composed of a mixture of longchain and short-chain lipidic components [33, 34].
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