Abstract
A sticky situation: Domain-dependent recognition of the glycosphingolipid galactosylceramide by norovirus-like particles (see picture; red/yellow) is shown using supported lipid bilayers (purple) as model membranes. Optimal ligand presentation is found to promote strong binding to GalCer. This presentation can be found at the edges of the glycosphingolipid-enriched domains (green) and binding is repressed in the absence of these domains.
Highlights
In-depth understanding of the cell-surface dependent processes leading to virus binding and infection of host cells, including the identification of new receptors mediating the initial steps, is of central importance for the development of new anti-viral therapies
Because of the positive interaction between the norovirus virus-like particles (VLPs) and GalCer, we extended the investigation to include VLP binding to GalCer mixed with 1-palmitoyl-2oleoyl-sn-glycero-3-phosphocholine (POPC) lipids in planar supported lipid bilayers (SLBs)
We further tested VLP binding to a bilayer containing 10 % GalCer labeled with the dye Atto647N by acyl-chain replacement
Summary
In-depth understanding of the cell-surface dependent processes leading to virus binding and infection of host cells, including the identification of new receptors mediating the initial steps, is of central importance for the development of new anti-viral therapies. Hççk Department of Applied Physics, Chalmers University of Technology SE-41133 Gçteborg (Sweden) E-mail: bally@chalmers.se Larson Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy, University of Gothenburg (Sweden) E-mail: goran.larson@clinchem.gu.se
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