Abstract
Herein we report a NADH sensor platform based on the gold nanoparticles (AuNPs) decorated electrochemically reduced graphene oxide (ERGO) stabilized with poly(allyamine hydrochloride) (PAH). The ERGO was obtained by direct electrochemical reduction of graphene oxide onto the electrode surface. The electrode material was characterized by Raman spectroscopy, Scanning Electron Microscopy and UV–vis spectrometry. The composite AuNPs–ERGO–PAH promotes the electron transfer between NADH and the modified screen-printed electrode surface at +510mV with a high sensitivity of 131.0±1.2μAmM−1cm2 and a detection limit of 3.5μM in amperometric measurements. The differential pulse voltammetry allowed the selective NADH detection in the presence of ascorbic acid, dopamine, glucose and uric acid. The AuNPs–ERGO–PAH/SPE sensing platform represents a promising starting point for development of dehydrogenase-based biosensors.
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