Abstract
The electrochemical biosensing efficiency of uric acid (UA) detection on an electrochemically reduced graphene oxide (ERGO)‐decorated electrode surface was studied by using various amine linkers used to immobilize ERGO. The amine linkers aminoethylphenyldiazonium, 2,2′‐(ethylenedioxy)bis(ethylamine), 3‐aminopropyltriethoxysilane, and polyethyleneimine were coated on indium–tin–oxide electrode surfaces through chemical or electrochemical deposition methods. ERGO‐decorated surfaces were prepared by the electrochemical reduction of graphene oxide (GO), which was immobilized on the amine‐coated electrode surfaces through the electrostatic interaction between GO and the ammonium ion of the linker on the surface. We monitored the sensing results of electrochemical UA detection with differential pulse voltammetry. The ERGO‐modified surface presented electrocatalytic oxidation of UA and ascorbic acid. Among the different amines tested, 3‐aminopropyltriethoxysilane provided the best biosensing performance in terms of sensitivity and reproducibility.
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