エンドウ品種アラスカの葉外植片からのカルス誘導と植物体再生

Abstract

Callus tissue was induced from pea leaf-explants on a Murashige-Skoog (MS) medium containing 2, 4-D (0.01-2.0mg/l) and kinetin (0.1-2.0mg/l). Adventitious roots and green spots were formed on the same medium within 40-days of culture. Shoot formation from green spots and subsequent growth were observed when the excised green spots were transferred to a fresh MS medium containing IAA (0.01, 0.04, 0.05mg/l) and kinetin (0.1, 0.5, 0.6mg/l).