Mutation Identification in the Complete Myostatin Sequence in Indonesian Kampung Chicken

Abstract

Kampung chicken, an Indonesian native chicken, has a potential for meat producers to improve national food security, as well as supplying highly favored meat to Indonesian consumers. To improve the meat production of Kampung chicken, the selection at the genomic level could be a promising approach. Myostatin is well known as a negative regulator of skeletal muscle growth. The objective of this study was to identify DNA mutation in the complete sequence of the myostatin gene in Kampung chicken. Genotyping was performed in 6 Kampung chickens by PCR and direct sequencing using 24 primer pairs covering 8.3 Kb of complete myostatin sequence. A total of 95 mutations discovered in the myostatin gene, including 24 in promoter, in 5’UTR, 5 in exon 1, 19 in intron 1, 37 in intron 2, 1 in exon 3, and 8 in 3’UTR. Among all mutations, 93 mutations were classified as point mutations, and 2 mutations were classified as indels. A total of 88 of 95 mutations (92%) were novel mutations. Six point mutations were found in the coding region, including 5 SNPs in exon 1, 1 SNP in exon 3, and no mutations in exon 2. No amino acid changes within all SNPs in coding region. Further studies in a larger population are needed to confirm this potential and novel mutations and their association with growth and meat production of Kampung chicken.