Muscle-specific transgenic expression of porcine myostatin propeptide enhances muscle growth in mice

Abstract

Myostatin is a well-known negative regulator of skeletal muscle growth. Inhibition of myostatin activity results in increased muscle mass. Myostatin propeptide, as a myostatin antagonist, could be applied to promote meat production in livestock such as pigs. In this study, we generated a transgenic mouse model expressing porcine myostatin propeptide under the control of muscle-specific regulatory elements. The mean body weight of transgenic mice from a line expressing the highest level of porcine myostatin propeptide was increased by 5.4% (P=0.023) and 3.2% (P=0.031) in males and females, respectively, at 8weeks of age. Weight of carcass, fore limb and hind limb was respectively increased by 6.0% (P=0.038), 9.0% (P=0.014), 8.7% (P=0.036) in transgenic male mice, compared to wild-type male controls at the age of 9weeks. Similarly, carcass, fore limb and hind limb of transgenic female mice was 11.4% (P=0.002), 14.5% (P=0.006) and 14.5% (P=0.03) respectively heavier than that of wild-type female mice. The mean cross-section area of muscle fiber was increased by 17% (P=0.002) in transgenic mice, in comparison with wild-type controls. These results demonstrated that porcine myostatin propeptide is effective in enhancement of muscle growth. The present study provided useful information for future study on generation of transgenic pigs overexpressing porcine myostatin propeptide for improvement of muscle mass.