Abstract
Mung bean is nutritious and rich in protein (19.5%–33.1%). However, there are few studies on mung bean protein active peptides so the mung bean protein hydrolysates (MBPHs) were investigated for evaluating their ability to clear intracellular reactive oxygen species (ROS) and regulating the ability of antioxidant enzymes on NCTC-1469 cells. Results showed that MBPHs, MBPHs-I (molecular weight < 3 kDa), MBPHs-II (molecular weight between 3 and 10 kDa), and MBPHs-III (molecular weight > 10 kDa) could all improve the survival rate of cells compared with the model group. MBPHs, MBPHs-I, and MBPHs-II could significantly decrease the content of lactate dehydrogenase (LDH) and reduce the generation of malonaldehyde (MDA) at a concentration of 0.4 mg/mL. Regarding the intracellular ROS, the result showed that MBPHs-I significantly reduced the production of ROS (from 58.3% to 26.6%) and had a dose-dependent relationship. In addition, the amino acid analysis showed that MBPHs-I had a balanced amino acid composition. MBPHs-I is rich in lysine but was deficient in cereals. Therefore, the hydrophobic and aromatic amino acids in MBPHs-I were high, which could improve its antioxidant activity. According to the results, MBPHs-I was the best and most potent natural antioxidant and it can contribute to drug development and medical application.
Highlights
Reactive oxygen species (ROS), including hydroxyl radicals (OH), peroxyl radicals (ROO), superoxide radicals (O2 ), hydrogen peroxide (H2 O2 ), and singlet oxygen [1], play a crucial role in human health and are an indispensable part of the cell of living organism composition [2].When ROS are at a low level, ROS can effectively play several beneficial roles in the human body, for example serving as an intracellular signaling factor
The activity of the NCTC-1469 cells is evaluated using CCK-8 analysis, and the results showed the number of living cells gradually decreased when the concentration and time of H2 O2 increased in
mung bean protein hydrolysates (MBPHs) were obtained by the enzymatic hydrolysis of mung bean protein and three components, MBPHs-I, MBPHs-II, and MBPHs-III, were isolated and purified by ultrafiltration membranes
Summary
When ROS are at a low level, ROS can effectively play several beneficial roles in the human body, for example serving as an intracellular signaling factor. Tissues, organs, and some of the biological macromolecules will be damaged by oxidative stress when ROS excesses the receptivity of the human body [3]. Some serious diseases, such as diabetes [4], neurodegenerative disease [5], cancers [6], and other cardiovascular diseases [7] were related to excessive oxidative stress reaction on the human body induced by ROS. SOD could remove free radicals and reduce oxidative
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