Abstract
MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections MEPS 410:25-41 (2010) - DOI: https://doi.org/10.3354/meps08649 Multi-scale spatial variation in stable isotope and fatty acid profiles amongst temperate reef species: implications for design and interpretation of trophic studies M. A. Guest1, A. J. Hirst1,2,*, P. D. Nichols3,4, S. D. Frusher1 1Tasmanian Aquaculture and Fisheries Institute, University of Tasmania, Hobart, Tasmania, Australia 2Marine and Freshwater Fisheries Research Institute, Queenscliff, Victoria, Australia 3CSIRO Marine and Atmospheric Research, Hobart, Tasmania, Australia 4Antarctic and Climate Ecosystems, University of Tasmania, Hobart, Tasmania, Australia *Corresponding author. Email: alastair.hirst@dpi.vic.gov.au ABSTRACT: Stable isotopes of carbon and nitrogen and fatty acid analyses are increasingly being used in combination to determine the trophic structure of marine systems. For stable isotopes, the variability in carbon and nitrogen isotopic signatures has long been recognised and has been characterised for some taxa. Whilst it is known that metabolic processes may influence fatty acid profiles, the spatial variability of fatty acid profiles has not been documented. Understanding at what scale these 2 biochemical tracers vary, and if the scale of variability corresponds between tracers, is crucial for the correct design and interpretation of combined tracers in trophic studies. This study is the first to examine spatial variability in fatty acid profiles per se, and in combination with stable isotope ratios in the same organisms at multiple spatial scales. We used a spatially hierarchical design which sampled across broad geographic regions, reefs within regions, and also between different parts of macroalgal plants common on temperate reefs. For stable isotopes of carbon and nitrogen, variability was greatest at intermediate spatial scales (between locations within regions, and sites within locations). In contrast, fatty acid profiles showed the greatest variation amongst individual replicates of lobster, abalone and macroalgae. This study demonstrates that for the increasing number of trophic studies using combined biochemical tracers, sampling design should cater to the differences in the variability of each tracer technique and allocate sampling accordingly. KEY WORDS: Stable isotopes · Fatty acid analysis · Nested hierarchical design · Multivariate variance components · Tasmania Full text in pdf format PreviousNextCite this article as: Guest MA, Hirst AJ, Nichols PD, Frusher SD (2010) Multi-scale spatial variation in stable isotope and fatty acid profiles amongst temperate reef species: implications for design and interpretation of trophic studies. Mar Ecol Prog Ser 410:25-41. https://doi.org/10.3354/meps08649 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in MEPS Vol. 410. Online publication date: July 14, 2010 Print ISSN: 0171-8630; Online ISSN: 1616-1599 Copyright © 2010 Inter-Research.
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