Abstract
Malaysia is the largest exporter of Elaeis Guineensis (Palm oil) in the international market. Oil palm cultivation generates a significant amount of lignocellulosic biomass derived from empty fruit bunches (EFB) as waste product. This research focused on optimizing the mycelium growth in Pleurotus sp. cultivation by using EFB as a culture medium. The EFB was cut into the range of size of substrate (S) from 1.5 cm to 3.0 cm, soaked in water for overnight, applied steam treatment and incubated at the selected range of temperature (T) from 29 °C to 32 °C. The responses were mycelium extension rate (M) and nitrogen concentration in mycelium (N). The multi-objective optimisation of M and N requires the objective functions which represent both processes. For this type of problem, multi-objective genetic algorithm was chosen as the methodology, specifically using NSGA-II algorithm. Through the implementation of selected multi-objective genetic algorithm, it was able to produce the pareto front for optimising both nitrogen concentration and the extension rate of the mycelium. The highest nitrogen concentration and mycelium extension rate was from the result with crossover and mutation probability of 0.5 and 0.2. It produced 388.45 mg/L of nitrogen concentration and 0.370 cm/day of mycelium growth.
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