MP66-05 OXIDATIVE STRESS PROTEINS IDENTIFIED IN HUMAN SPERMATOZOA BY PROTEOMIC AND BIOINFORMATIC ANALYSIS

Abstract

You have accessJournal of UrologyInfertility: Basic Research, Physiology & Pathophysiology1 Apr 2014MP66-05 OXIDATIVE STRESS PROTEINS IDENTIFIED IN HUMAN SPERMATOZOA BY PROTEOMIC AND BIOINFORMATIC ANALYSIS Rakesh Sharma, Ashok Agarwal, Damayanthi Durairajanayagam, Zhihong Cui, Ahmet Ayaz, and Edmund Sabanegh Rakesh SharmaRakesh Sharma More articles by this author , Ashok AgarwalAshok Agarwal More articles by this author , Damayanthi DurairajanayagamDamayanthi Durairajanayagam More articles by this author , Zhihong CuiZhihong Cui More articles by this author , Ahmet AyazAhmet Ayaz More articles by this author , and Edmund SabaneghEdmund Sabanegh More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.2052AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Oxidative stress is associated with male infertility. Understanding the protein profile of spermatozoa is essential for better diagnosis of male infertility. The aim of the present study was to examine the effect of high ROS production on the proteomic profile and cellular distribution of spermatozoal proteins. METHODS Seminal ejaculates from 52 subjects (32 infertile men and 20 healthy men) were classified as ROS+ or ROS- and evaluated for their proteomic profile. Samples were pooled and subjected to LC-MS/MS analysis. Differentially (over- or under-) expressed proteins were identified and further categorized as common and unique. Functional bioinformatic analysis was done using both publicly available Gene Ontology annotations, as well as proprietary databases such as Ingenuity Pathway Analysis (IPATM) and MetacoreTM to identify the differentially affected processes, pathways, interactions and cellular distribution of the proteins. RESULTS Of 74 identified proteins, 47 were over-expressed and 27 under-expressed compared to the ROS- group. Fifteen of the 74 proteins were 2-fold differentially expressed in the ROS+ group, of which 10 were overexpressed and 5 underexpressed. Most of the differentially expressed proteins were localized to the cytoplasmic and intracellular regions. In the ROS+ group, there was increased expression of histone cluster 1H2ba, mitochondrial malate dehydrogenase precursor, transglutaminase 4, glutathione peroxidase 4 isoform A precursor, glutamine synthetase and heat shock proteins. Androgen receptor was identified as one of the topmost regulators in the ROS+ group with 21 differentially expressed proteins interacting with the receptor. cAMP responsive element modulator (CREM) signaling, which plays an important role in male fertility, was one of the key pathways associated with the differentially expressed proteins. CONCLUSIONS We identified various proteins that are implicated in a multitude of functions associated with response and management of oxidative stress. The increased expression of proteins in the ROS+ group suggests some of these proteins may serve as potential biomarkers of oxidative stress. Alterations reported in our study may help explain pathways leading to the altered semen phenotype, especially in men exhibiting oxidative stress. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e742 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Rakesh Sharma More articles by this author Ashok Agarwal More articles by this author Damayanthi Durairajanayagam More articles by this author Zhihong Cui More articles by this author Ahmet Ayaz More articles by this author Edmund Sabanegh More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...