Abstract
Purpose: CREM (cyclic AMP-responsive element modulator) gene is essential for spermatogenesis, as it is necessary in differentiation of spermatids into sperm. In this study, to investigate the effect of Epimedii Herba (EH) on the sperm functions and the CREM transcription factor expressions in cyclophosphamide-treated mouse testes, C57BL/c mice were divided into five groups, the normal group, CP only-treated group and EH with CP (100, 500, 1000mg/kg of EH and 100mg/kg of CP) treated group for five weeks. Methods: We performed semi-quantitive PCR and western blot analysis for the examination of the CREM expression and analyzed sperm parameters using CASA system. Results: In our results, EH showed protective effect on CP induced reproductive toxicity in male mice by recovering sperm count, motility. CREM expression increased in EH treated groups at 500mg/kg concentration than that of control group (83.8%; p<0.01 vs. 71.5%; p<0.05, respectively). Conclusion:Our results suggest that EH plays an important role in male fertility, and it could be applied for clinical use in infertility treatment. Contact: Kyujin Chung, hykim0927@naver.com
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