MP03-17 L-γ-GLUTAMYLETHYLAMIDE PREVENTS LOWER URINARY TRACT DYSFUNCTION INDUCED BY CHRONIC SYMPATHETIC HYPERACTIVITY

Abstract

You have accessJournal of UrologyCME1 Apr 2023MP03-17 L-γ-GLUTAMYLETHYLAMIDE PREVENTS LOWER URINARY TRACT DYSFUNCTION INDUCED BY CHRONIC SYMPATHETIC HYPERACTIVITY Kanako Matsuoka, Kei Yaginuma, Yu Endo, Yuki Harigane, Yuta Matsuoka, Yuki Yoshida, Hitomi Imai, Akifumi Onagi, Seiji Hoshi, Tomoyuki Koguchi, Junya Hata, Yuichi Sato, Hidenori Akaihata, Masao Kataoka, and Yoshiyuki Kojima Kanako MatsuokaKanako Matsuoka More articles by this author , Kei YaginumaKei Yaginuma More articles by this author , Yu EndoYu Endo More articles by this author , Yuki HariganeYuki Harigane More articles by this author , Yuta MatsuokaYuta Matsuoka More articles by this author , Yuki YoshidaYuki Yoshida More articles by this author , Hitomi ImaiHitomi Imai More articles by this author , Akifumi OnagiAkifumi Onagi More articles by this author , Seiji HoshiSeiji Hoshi More articles by this author , Tomoyuki KoguchiTomoyuki Koguchi More articles by this author , Junya HataJunya Hata More articles by this author , Yuichi SatoYuichi Sato More articles by this author , Hidenori AkaihataHidenori Akaihata More articles by this author , Masao KataokaMasao Kataoka More articles by this author , and Yoshiyuki KojimaYoshiyuki Kojima More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003214.17AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Chronic sympathetic hyperactivity had been reported as a factor of lower urinary tract dysfunction (LUTD), but little is known about the mechanisms. L-γ-glutamylethylamide (L-theanine), one of the glutamine derivatives in Japanese green tea, has recently focused on the sympathetic inhibitory effects. Hence, we speculated that L-theanine might suppress the development of LUTD associated with chronic sympathetic hyperactivity. This study aimed to clarify the mechanisms underlying the effect of L-theanine on LUTD in spontaneously hypertensive rats (SHR) known as one of OAB rat models with chronic sympathetic hyperactivity. METHODS: Twelve-week-old SHR were divided into two groups (controls, n=10; L-theanine, n=10). The L-theanine group was given only L-theanine solution to drink freely. After six weeks, blood pressure, plasma catecholamine level measurement, and cystometry were performed in both groups. The bladder tissue was harvested for pharmacological studies, histological examinations, and protein expression analysis. Contractile responses to 80-mM KCl, electrical field stimulation, 1-mM ATP, and carbachol in organ baths were recorded. Malondialdehyde (MDA), biomarkers for oxidative stress, was assessed using immunohistochemical staining and Western blotting. Values of p<0.05 were considered statistically significant. RESULTS: There was no significant difference in systolic and diastolic blood pressure between two groups. Mean blood pressure was significantly lower in the L-theanine group than in controls (p=0.046). The plasma noradrenaline level was significantly lower in the L-theanine group (365.5±31.6 pg/ml) than in controls (246.2±34.3 pg/ml, p=0.04). The cystometrogram showed the micturition interval and mean voided volume were significantly larger in the L-theanine group (7.8±1.7 min, 1.3±0.35 ml) than in controls (5.6±8.4 min, 0.96±0.18 ml, p=0.002, p=0.03). Contractile responses of bladder strip were significantly higher in the L-theanine group than in controls. The collagen/muscle ratio of the bladder smooth muscle was significantly decreased in the L-theanine group (p=0.02). The expression of MDA was significantly decreased in the L-theanine group (p<0.001). CONCLUSIONS: These results suggested that L-theanine ameliorated excessive contraction of vascular smooth muscle in peripheral small arteries by suppressing elevated serum levels of noradrenaline in SHR, thereby decreasing MBP. Oral administration of L-theanine for 6 weeks may contribute to the prevention of bladder voiding function, along with storage function by increasing blood perfusion in the bladder. Source of Funding: Nothing © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e30 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Kanako Matsuoka More articles by this author Kei Yaginuma More articles by this author Yu Endo More articles by this author Yuki Harigane More articles by this author Yuta Matsuoka More articles by this author Yuki Yoshida More articles by this author Hitomi Imai More articles by this author Akifumi Onagi More articles by this author Seiji Hoshi More articles by this author Tomoyuki Koguchi More articles by this author Junya Hata More articles by this author Yuichi Sato More articles by this author Hidenori Akaihata More articles by this author Masao Kataoka More articles by this author Yoshiyuki Kojima More articles by this author Expand All Advertisement PDF downloadLoading ...