Abstract
TREATMENT of cultured mammalian cells with cytochalasin B, a metabolite isolated from the mould Helminthosporium dematioideum, yields a population of enucleated cells1–3. Such enucleates are excellent experimental material for the study of nucleus-cytoplasm interactions as certain normal physiological characteristics of the intact cells can be preserved. Indeed, it has been reported that baby hamster kidney cells (BHK-21) and African green monkey kidney cells (BSC-1) enucleated with cytochalasin retain such properties of their intact counterparts as cell shape formation, cell locomotion and contact inhibition4. Furthermore, replication of vaccinia virus5 and polio virus6 can occur in enucleates from mouse L cells and BSC-1 cells, respectively.
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