Abstract
THE enzyme system known as nitrogenase is responsible for conversion of elementary nitrogen in microbial-plant symbiotic nitrogen fixing associations. A method has been developed for the isolation of nitrogenase mutants of Rhizobium meliloti for the study of genes regulating the synthesis of nitrogenase. This type of ineffective mutant cannot be selected directly since nitrogenase is only synthesised in bacteroids within the plant host.
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