Molecular Screening of β-Lactoglobulin Gene Variation for Allergy-free Milk Production in Indonesian Friesian Holstein

Abstract

The most allergenic protein in cow’s milk for new-borns is Beta Lactoglobulin (β-LG), which is also one of the bovine whey proteins. As a result, choosing suitable animals to create free allergenic protein is essential. In this study, Indonesian Friesian Holstein’s genetic variants in the β-LG gene were examined). Forty-two blood samples were sampled as DNA sources. The β-LG gene allele and genotype variant were identified using PCR-RFLP, or Polymerase Chain Reaction - Restriction Fragment Length Polymorphism. Successful amplification of the β-LG gene with a 247 bp product size was followed by DNA sequencing and HaeIII enzyme digestion (GG|CC). There were discovered to be three genotypes and two types of alleles, A and B, with the frequencies being AA (0.26), AB (0.50), and BB (0.24). The population is in equilibrium because A and B have respective allele frequencies of 0.51 and 0.49. In conclusion, molecular-assisted selection can create allergen-free milk by utilizing the genotype variants of β-LG genes.