Deoxyribonucleic acid analysis of trophoblastic tumors

Abstract

To the Editors:We read with great interest the article by Fisher and Newlands (Fisher RA, Newlands ES. Rapid diagnosis and classification of hydatidiform moles with polymerase chain reaction. AM J OBSTET GYNECOL 1993;168:563-9). They reported that with polymerase chain reaction they were able to effectively diagnose and classify hydatidiform moles; nine of 13 complete hydatidiform moles were shown to be androgenetic and two of the complete hydatidiform moles were classified as dispermic.Even in the case in which androgenesis of molar tissue cannot be established by polymerase chain reaction, however, deoxyribonucleic acid (DNA) fingerprinting confirms the paternal origin of complete mole as Fisher and Newlands described. We have been focusing our efforts for the application of DNA fingerprinting and polymerase chain reaction to the pathogenesis of trophoblastic tumors. By the use of minisatellite probe obtained from Jeffreys (University of Leicester, U.K.), we determined androgenesis as a cause of complete hydatidiform mole.1Saji F Tokugawa Y Kimura T et al.A new approach using DNA fingerprinting for the determination of androgenesis as a cause of hydatidiform mole.Placenta. 1989; 10: 399-405Abstract Full Text PDF PubMed Scopus (22) Google Scholar We also used a gene amplification method by using polymerase chain reaction for the restriction fragment length polymorphism analysis of extracellular DNA (mitochondrial DNA) of complete hydatidiform mole. The restriction fragment length polymorphism of molar mitochondrial DNA coincided with those of patient, indicating that complete mole results from the fertilization of an anuclear ovum with normal sperm.2Azuma C Saji F Tokugawa Y et al.Application of gene amplification by polymerase chain reaction to genetic analysis of molar mitochondrial DNA: the detection of anuclear empty ovum as the cause of complete mole.Gynecol Oncol. 1991; 40: 29-33Abstract Full Text PDF PubMed Scopus (54) Google ScholarWe studied the pathogenesis of choriocarcinoma by DNA analysis of restriction fragment length polymorphism, and the results obtained suggested that the pathogenesis of choriocarcinoma varies, being completely differed from that of the complete hydatidiform mole in some cases; the patterns of the polymorphic fragments in tumor tissue were not always paternal origin but identical to those in the host DNA in some cases.3Azuma C Saji F Nobunaga T et al.Studies on the pathogenesis of choriocarcinoma by analysis of restriction fragment length polymorphisms.Cancer Res. 1990; 50: 488-491PubMed Google Scholar It is very interesting if Fisher et al. analyzed DNA polymorphism of choriocarcinoma by the use of polymerase chain reaction and got results similar to ours. To the Editors:We read with great interest the article by Fisher and Newlands (Fisher RA, Newlands ES. Rapid diagnosis and classification of hydatidiform moles with polymerase chain reaction. AM J OBSTET GYNECOL 1993;168:563-9). They reported that with polymerase chain reaction they were able to effectively diagnose and classify hydatidiform moles; nine of 13 complete hydatidiform moles were shown to be androgenetic and two of the complete hydatidiform moles were classified as dispermic.Even in the case in which androgenesis of molar tissue cannot be established by polymerase chain reaction, however, deoxyribonucleic acid (DNA) fingerprinting confirms the paternal origin of complete mole as Fisher and Newlands described. We have been focusing our efforts for the application of DNA fingerprinting and polymerase chain reaction to the pathogenesis of trophoblastic tumors. By the use of minisatellite probe obtained from Jeffreys (University of Leicester, U.K.), we determined androgenesis as a cause of complete hydatidiform mole.1Saji F Tokugawa Y Kimura T et al.A new approach using DNA fingerprinting for the determination of androgenesis as a cause of hydatidiform mole.Placenta. 1989; 10: 399-405Abstract Full Text PDF PubMed Scopus (22) Google Scholar We also used a gene amplification method by using polymerase chain reaction for the restriction fragment length polymorphism analysis of extracellular DNA (mitochondrial DNA) of complete hydatidiform mole. The restriction fragment length polymorphism of molar mitochondrial DNA coincided with those of patient, indicating that complete mole results from the fertilization of an anuclear ovum with normal sperm.2Azuma C Saji F Tokugawa Y et al.Application of gene amplification by polymerase chain reaction to genetic analysis of molar mitochondrial DNA: the detection of anuclear empty ovum as the cause of complete mole.Gynecol Oncol. 1991; 40: 29-33Abstract Full Text PDF PubMed Scopus (54) Google ScholarWe studied the pathogenesis of choriocarcinoma by DNA analysis of restriction fragment length polymorphism, and the results obtained suggested that the pathogenesis of choriocarcinoma varies, being completely differed from that of the complete hydatidiform mole in some cases; the patterns of the polymorphic fragments in tumor tissue were not always paternal origin but identical to those in the host DNA in some cases.3Azuma C Saji F Nobunaga T et al.Studies on the pathogenesis of choriocarcinoma by analysis of restriction fragment length polymorphisms.Cancer Res. 1990; 50: 488-491PubMed Google Scholar It is very interesting if Fisher et al. analyzed DNA polymorphism of choriocarcinoma by the use of polymerase chain reaction and got results similar to ours. We read with great interest the article by Fisher and Newlands (Fisher RA, Newlands ES. Rapid diagnosis and classification of hydatidiform moles with polymerase chain reaction. AM J OBSTET GYNECOL 1993;168:563-9). They reported that with polymerase chain reaction they were able to effectively diagnose and classify hydatidiform moles; nine of 13 complete hydatidiform moles were shown to be androgenetic and two of the complete hydatidiform moles were classified as dispermic. Even in the case in which androgenesis of molar tissue cannot be established by polymerase chain reaction, however, deoxyribonucleic acid (DNA) fingerprinting confirms the paternal origin of complete mole as Fisher and Newlands described. We have been focusing our efforts for the application of DNA fingerprinting and polymerase chain reaction to the pathogenesis of trophoblastic tumors. By the use of minisatellite probe obtained from Jeffreys (University of Leicester, U.K.), we determined androgenesis as a cause of complete hydatidiform mole.1Saji F Tokugawa Y Kimura T et al.A new approach using DNA fingerprinting for the determination of androgenesis as a cause of hydatidiform mole.Placenta. 1989; 10: 399-405Abstract Full Text PDF PubMed Scopus (22) Google Scholar We also used a gene amplification method by using polymerase chain reaction for the restriction fragment length polymorphism analysis of extracellular DNA (mitochondrial DNA) of complete hydatidiform mole. The restriction fragment length polymorphism of molar mitochondrial DNA coincided with those of patient, indicating that complete mole results from the fertilization of an anuclear ovum with normal sperm.2Azuma C Saji F Tokugawa Y et al.Application of gene amplification by polymerase chain reaction to genetic analysis of molar mitochondrial DNA: the detection of anuclear empty ovum as the cause of complete mole.Gynecol Oncol. 1991; 40: 29-33Abstract Full Text PDF PubMed Scopus (54) Google Scholar We studied the pathogenesis of choriocarcinoma by DNA analysis of restriction fragment length polymorphism, and the results obtained suggested that the pathogenesis of choriocarcinoma varies, being completely differed from that of the complete hydatidiform mole in some cases; the patterns of the polymorphic fragments in tumor tissue were not always paternal origin but identical to those in the host DNA in some cases.3Azuma C Saji F Nobunaga T et al.Studies on the pathogenesis of choriocarcinoma by analysis of restriction fragment length polymorphisms.Cancer Res. 1990; 50: 488-491PubMed Google Scholar It is very interesting if Fisher et al. analyzed DNA polymorphism of choriocarcinoma by the use of polymerase chain reaction and got results similar to ours.