Abstract
The molecular diversity of Begomovirus infecting Horsegram yellow Mosaic viruses (HgYMV1 and HgYMV2), from two main horsegram growing farms near Bangalore, Karnataka State, South India was investigated. The viral DNA was amplified from horsegram plants exhibiting mild and severe symptoms by polymerase chain reaction, and complete genome of the HgYMV were identified by their sequence analysis. Isolates of HgYMV1 and HgYMV2 were found to be associated with severe symptom phenotype from HgYMV. HgYMV was most closely related to Mungbean yellow mosaic indian viruse (MYMIV) and Mungbean yellow mosaic virus (MYMV) at 81.8 to 84.8 % nucleotide identity, based on DNA-A and DNA-B component sequences. HgYMV was distantly related to Dolicos yellow mosaic virus from Asia (DoYMV-Ban and DoYMV-DB) and partially to Cowpea golden mosaic virus from Nigeria (CPGMV-[NG]) at 64 and 62 % DNA nucleotide identity. Analysis of the DNA-B sequence of HgYMV revealed a DNA-B component identical to those of Bean golden yellow mosaic virus BGYMV isolates described. Furthermore, the DNA-B component for extant BGYMV isolates and strains were also the closest relatives for the HgYMV1 DNA-B components at 48.7 % nucleotide identity. Therefore, HgYMV could be considered to be a new species of the genus Begomovirus (family Geminiviridae). Keywords: Begomovirus, Horsegram, yellow Mosaic viruses , DNA sequencing
Highlights
Horsegram (Macrotyloma uniflorum) is a small pulse that produces seeds, and one of the lesser known beans
This study reports the sequence of the Begomovirus causing Horsegram leaf curl in India
Leaves of Horsegram plant showing symptoms typical of yellow mosaic disease were collected from -80 oC freezer at Natural Resources Institute, University of Greenwich, United Kingdom
Summary
Horsegram (Macrotyloma uniflorum) is a small pulse (legume) that produces seeds, and one of the lesser known beans. Horsegram is an annual native to South Asia It is regarded as a food of the poor, in southern India. It has been linked to reduced risk of various diseases due to presence of phtyochemicals such as tannins and phytate (Kawsar et al, 2008; Sreerama et al, 2012, Prasad and Singh, 2015). These phtyochemicals are thought to have beneficial medicinal and nutraceutical properties (Muthukumara et al, 2014; Bhartiya et al, 2015)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
