Abstract
Objective To identify the etiology of an outbreak of cute hemorrhagic conjunctivitis (AHC) occurred in Hubei in 2010 and analyze the molecular evolution of these viruses. Methods Conjunctival swabs from 20 eyes were collected from AHC patients, performed virus isolation was performed and then these specimens were tested for human enterovirus 70(EV-D70) and Coxsackievirus A24 variant(CV-A24v) using polymerase chain reaction (PCR) technique. All CV-A24v isolates were subjected to 3C and VP1 coding region sequencing and phylogentic tree were constructed for Hubei CV-A24v and other CV-A24v worldwide based on 3C and VP1 region, respectively. Results The results showed that 11 out of 20 specimens were positive for virus isolation and identified as CV-A24v. The phylogenetic tree based on 3C region showed Hubei CV-A24v belong to Cluster 3 in Genotype Ⅳ(GⅣ-C3)with strains isolated throughout the world after 2010 and further divided into A and B lineages. Conclusions This study suggested that at least two transmission lineages circulated in HuBei province in 2010 causing the AHC outbreak nationwide. Key words: Coxsackievirus infections; Enterovirus B, human; Conjunctivitis, acute hemorrhagic; Phylogeny; Genes
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