MOLECULAR GENETIC AND FUNCTIONAL ANALYSIS OF GENES INFLUENCING PYOVERDINE SYNTHESIS IN PSEUDOMONAS BRASSICACEARUM S-1

Abstract

Molecular genetic and functional analysis allowed the identification and characterization of a number of new genetic
 determinants affecting the synthesis of pyoverdine by P. brassicacearum strain S-1. It was established that inactivation
 of genes encoding Zn-dependent peptidase (mtfA), peptidase of the C39 (GFU70_09550) family, transmembrane sensor
 protein (bvgS), heme transporter (ccmC), and protein with unknown function (ydgA) led to changes in fluorescence,
 efficacy of pyoverdine synthesis and antimicrobial activity in P. brassicacearum strain S-1. A previously undescribed
 genetic locus that determines the synthesis of pyoverdin was found in the chromosome of the studied bacteria. It
 was shown that the directed inactivation of this locus led to the complete absence of fluorescence and the production
 of pyoverdine by P. brassicacearum strain S-1, as well as to the loss of the ability to suppress the growth of the
 phytopathogenic strain Fusarium oxysporum BIM F-798.