Molecular epidemiological survey on seven viral pathogens in children with diarrhea disease in Urumqi area in 2011

Abstract

Objective To study the infection status of seven viral pathogens in children under 5-years old with diarrhea in Urumqi in 2011 and their epidemiological characteristics. Methods A total of 315 fecal samples were collected from in-patient and out-patient children 5 years and younger with diarrhea from January to December of 2011 in Urumqi. Polymerase chain reaction （PCR） was used to detect adenovirus （AdV）, and multiplex reverse transcription-polymerase chain reaction （RT-PCR） was performed to determine rotavirus （RV）, aichi virus （AIV）, human parechovirus （HPeV）, human calicivirus（ HuCV）, enterovirus （EV） and astrovirus （AstV）. Results The positive rates of RV, AIV, HPeV, HuCV, EV, AdV and AstV in 315 fecal samples were 27.30% （86/315）, 18.41% （58/315）, 12.38% （39/315）, 12.06% （38/315）, 5.08% （ 16/315 ）, 3.81% （ 12/315 ） and 0.00% respectively, moreover, mixed infection was detected in 54 of 315 samples （ 17.14% ）. Children under 2-years old accounted for most of the patients with above mentioned infec- tion. Seasonal differences were found in infections of the six viruses, which were characterized by RV infection was mainly occurred in autumn and winter, AIV infection most in the latter half of the year, HPeV, HuCV and EV infection in summer, while AdV infection had no seasonal fluctuation. Conclusion Rotavirus was the pre- dominant pathogen causing infant diarrhea in Urmuqi, followed by AIV, HPeV and HuCV, moreover, EV and AdV infection also played an unignorable role in the infantile diarrhea. Key words: Children; Diarrhea; Diarrhea virus; Diarrhea-related virus