Molecular detection of the virulence gene’s VacA and CagA of Helicobacter pylori by PCR

Abstract

Helicobacter pylori has several virulence factors, including the ability to produce urease, which allows it to colonize the stomach and survive for long periods of time. As a results of those virulence factors of H. Pylor i causes gastritis. This study aimed to detect H. pylori using of real-time PCR amplification of VacA and CagA genes, and compare between both genes and detection by urease and creatine. For this purpose, a total of 50 patients were selected and have been clinically diagnosed with gastritis. Biopsy samples were collected from all the patients. Rapid urease test (RUT) was applied to all gastric samples to detect the presence of H. Pylori . DNA was extracted and the RT-PCR was perform to detect the VacA and CagA genes. The results of RUT showed 20 samples were positive of H. Pylori , after the Real Time-PCR test were done the results showed 27 (54%) to VacA and 25 (50%) to CagA genes were positives.