Molecular detection of some virulence factors of uropathogenic proteus mirabilis using PCR technique

Abstract

A total of 25 isolates of P. mirabilis are recovered from (300) patients suffering from urinary tract infections (isolation rate is 8.3%) using the bacteriological culture technique. This cross-sectional study was conducted in Diyala province and sample collection includes Al-Khalis General Hospital, Baquba Teaching Hospital, and Al-Batool Teaching Hospital for maternity and Children, during the period extended from 5/8/2020 to 25/1/2021. A total of (300) urine specimens were collected from patients clinically complaining of signs and symptoms of UTIs. The age range was 2-56 years. 16 (64%) were females and 9 (36 %) were males. The study was approved by the scientific committee in the Directorate of Health in Diyala province Human privacy was respected by taken patients' or their parent's verbal consent. Standard bacteriological culture on different culture media (Himedia, Germany). Isolation and identification of P. mirabilis were achieved by morphological, biochemical, and ViaTeK2 procedures. The study aimed to isolate and diagnose Proteus bacteria from patients with UTIs. Detection of the phenotype of specific virulence factors using culture methods. Molecular analysis of some virulence factors using polymerase chain reaction. These isolates underwent polymerase chain reaction (Promega, USA) to detect virulence genes including; Urec gene, Mrp gene, and FlaA gene Regarding the virulence factors; all isolates (100%) were positive for hemolysin, urease, siderophore, and colony-forming I, II, and III as detected by bacteriological and chemical procedures. The results found that all (100%) isolates had the Urec gene, Mrp gene, FlaA gene.