Molecular characterization of Proteus mirabilis virulence factors isolated from patients with otitis media in Diwaniyah, Iraq

Abstract

The current study's goal was to identify virulence factors (genes) in P. mirabilis. A total of 25/100 (25%) Proteus mirabilis were obtained from patients with otitis media and identified using culture and biochemical characteristics, and Polymerase chain reaction (PCR). The PCR technique was used to detect the 16S rRNA gene and all virulence factors, including the ureC gene, which encodes urease synthesis, the flaA gene, which encodes flagella and fimbriae production, the hpmA gene, which encodes hemolysin production, and the zapA gene, which encodes protease. The results revealed that Proteus mirabilis isolates have 100% of the 16S rRNA gene, 100% of the ureC gene, 95% of the flaA gene, 95% of the hpmA gene, and 100% of the zapA gene.