Abstract

This paper describes the effect of NF-κB and Nrf2 transcription factor modulators on the depolymerization of periodontal extracellular matrix biopolymers in the early post-traumatic period following the modeling of an experimental traumatic brain injury (TBI). The study was conducted on 30 white Wistar male rats weighing 180-220 g, divided into 6 groups: the 1st (pseudo-traumatized animals, control 1) the scalp of the rats was pinched with a Mikulicz clamp for one click (under ether anesthesia); the 2nd included animals after the experimental TBI modeling (control 2); rats of other groups after the TBI modeling were intraperitoneally injected with the following modulators of transcription factors for 7 days: ammonium pyrrolidine dithiocarbamate, an inhibitor of nuclear translocation of NF-κB, in a dose of 76 mg/kg, dimethyl fumarate, an inducer of the transcription factor Nrf2, in a dose of 15 mg/kg in a 10% solution of dimethyl sulfoxide, and bioflavonoids possessing NF-κB inhibitor and Nrf2 inducer properties, namely: epigallocatechin-3-gallate in a dose of 1 mg/kg and a water-soluble form of quercetin (corvitin) in a dose of 100 mg/kg (10 mg/kg in terms of quercetin). The study has shown that the TBI modeling manifests an increase in collagenolysis and depolymerization of proteoglycans and sialoglycoproteins in the periodontal soft and bone tissue at the end of the early post-traumatic period (on the 7th day) as evidenced by the growth in the concentration of free oxyproline, hexuronic acids and N-acetylneuraminic acid in the homogenate of these tissues. The administration of specific modulators of the transcription factors NF-κB and Nrf2 (ammonium pyrrolidine dithiocarbamate and dimethyl fumarate, respectively) and herbal bioflavonoids (epigallocatechin-3-gallate and quercetin) after modeling TBI significantly inhibits the processes of depolymerization of macromolecules in the connective tissue of periodontal soft and bone structures (collagen, proteoglycans, glycoproteins) with the release and increase in the concentration of their monomers (oxyproline, hexuronic and N-acetylneuraminic acids). The article compares the effectiveness of the use of specific modulators of transcription factors NF-κB and Nrf2 and bioflavonoids under experimental conditions.

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