MMP9 −1562 C/T POLİMORFİZMİ, TÜRK TİP 2 DİABETES MELLİTUS HASTALARINDA ARTMIŞ DİYABETİK RETİNOPATİ RİSKİ İLE İLİŞKİLİDİR

Abstract

Objective
 Type 2 diabetes mellitus (T2DM) and diabetic
 retinopathy (DR) development is affected by genetic
 factors. Matrix metalloproteinases (MMPs) are involved
 in many cellular processes, such as remodeling of
 extracellular matrix proteins and angiogenesis. In
 this study, we investigated the relationship between
 MMP9 −1562 C/T (rs3918242) polymorphism and the
 development of T2DM and DR in Turkish population.
 Material and Method
 A total of 510 participants, involving 168 T2DM
 cases with DR, 168 T2DM cases without DR,
 and 174 controls, were included in the study. The
 polymerase chain reaction-restriction fragment length
 polymorphism (PCR-RFLP) method was carried out
 to identify genotypes.
 Results
 The CT, CT+TT genotypes and the T allele of the
 MMP9 −1562 C/T polymorphism were associated with
 increased risk of DR (p=0.001). On the other hand,
 no relationship was found between the development
 of T2DM and this polymorphism. In addition,
 CT (p=0.010) and CT+TT (p=0.015) genotype
 frequencies were found to be higher in proliferative
 diabetic retinopathy (PDR) cases compared to nonproliferative
 diabetic retinopathy (NPDR) cases, but
 after regression analysis, only insulin use (p=0.003)
 was found to be associated with the development of
 PDR.
 Conclusion
 Our data show that the MMP9 −1562 C/T polymorphism
 is associated with the development of DR in Turkish
 T2DM patients, but no significant relationship was
 found between this polymorphism and the severity of
 DR and the development of T2DM.