MircoRNA-1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1.

Dong Mei Wu ,Dong‐Mei Wu,Yuan‐Lin Zheng,Xin Wen,Qun Shan,Shan Wang,Yong‐Jian Wang,Xin‐Rui Han,Zi‐Feng Zhang,Jun Lu,Shao‐Hua Fan,Zi Feng Zhang

doi:10.1111/jcmm.13760

Dong Mei Wu , Dong‐Mei Wu + Show 10 more

Open Access

https://doi.org/10.1111/jcmm.13760

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Abstract

This study was designed to explore the relationship between miR‐1275 and SERPINE1 and its effects on glioma cell proliferation, migration, invasion and apoptosis. Differentially expressed miRNAs and mRNAs in glioma tissues were screened out by bioinformatic analysis. Dual‐luciferase reporter gene assay was used to validate the targeted relationship between miR‐1275 and SERPINE1. qRT‐PCR was used to detect the expression of miR‐1275 and SERPINE1 in glioma tissues. The expressions of SERPINE1 and p53 pathway‐related proteins in glioma cells were detected by western blot. Glioma cell proliferation, apoptosis, migration and invasion were respectively detected by CCK‐8 assay, flow cytometry, wound healing assay and transwell assay. Tumour xenograft model was developed to study the influence of miR‐1275 and SERPINE1 on glioma growth in vivo. The results of microarray analysis, qRT‐PCR and western blot showed that miR‐1275 was low‐expressed while SERPINE1 was high‐expressed in glioma. Dual‐luciferase assay showed that miR‐1275 could bind to SERPINE1. Overexpression of miR‐1275 could promote the p53 pathway‐related proteins’ expression. Highly expressed miR‐1275 could repress the migration, proliferation and invasion of glioma cells while highly expressed SERPINE1 had inverse effects. Tumour xenograft showed that up‐regulated miR‐1275 or down‐regulated SERPINE1 could repress glioma growth in vivo. Up‐regulation of miR‐1275 activated p53 signalling pathway via regulating SERPINE1 and therefore suppressed glioma cell proliferation, invasion and migration, whereas promoted cell apoptosis.

Highlights

Glioma, a kind of malignancy arising from glial cells in the brain, is the most common malignant tumours occurring in the centralWu and Wang are regarded as co-first authors.nervous system and accounting for 78% of intracranial primary tumours.[1,2] Glioma usually exhibits a series of malignant features, including extensive, rapid and aggressive progression, resistance to surgery, radiotherapy and chemotherapy, all of which lead to the poor prognosis for most patients.[3]
It was reported that miR‐1275 exhibited its tumour suppressive role on nasopharyngeal carcinoma cells by down‐regulating oncogenic HOXB5, whose expression was negatively related to miR‐1275.25 In our study, we found that miR‐1275 was lowly expressed in glioma and its up‐regulation could suppress glioma cell function including proliferation, migration and invasion, as well as induce apoptosis
We found that miR‐1275 could bind to SERPINE1, which was highly expressed in glioma

Summary

| INTRODUCTION

A kind of malignancy arising from glial cells in the brain, is the most common malignant tumours occurring in the central. Nervous system and accounting for 78% of intracranial primary tumours.[1,2] Glioma usually exhibits a series of malignant features, including extensive, rapid and aggressive progression, resistance to surgery, radiotherapy and chemotherapy, all of which lead to the poor prognosis for most patients.[3] extensive efforts have been made to improve glioma treatment, average survival time of Our discovery might contribute to perceiving the in‐depth molecular mechanism of glioma tumorigenesis

| MATERIALS AND METHODS

| RESULTS

A Normal

Findings

| DISCUSSION

| CONCLUSION