Abstract
Line-field confocal optical coherence tomography (LC-OCT) is an imaging technique in which A-scans are acquired in parallel through line illumination with a broadband laser and line detection with a line-scan camera. B-scan imaging at high spatial resolution is achieved by dynamic focusing in a Linnik interferometer. This paper presents an LC-OCT device based on a custom-designed Mirau interferometer that offers similar spatial resolution and detection sensitivity. The device has the advantage of being more compact and lighter. In vivo imaging of human skin with a resolution of 1.3 µm × 1.1 µm (lateral × axial) is demonstrated over a field of 0.9 mm × 0.4 mm (lateral × axial) at 12 frames per second.
Highlights
Line-field confocal optical coherence tomography (LC-OCT) is a recently introduced interferometric imaging technique, based on the time-domain version of optical coherence tomography (OCT) [1], with line illumination of the sample and line detection of the signal instead of point scanning and point detection as in conventional OCT [2]
The focus is continuously adjusted during the scan of the sample depth, allowing the use of a high numerical aperture (NA) microscope objective to image with high lateral resolution
We have developed an LC-OCT device based on a custom-designed Mirau interferometer that o ers similar spatial resolution and detection sensitivity compared to the previously reported LC-OCT devices based on a Linnik interferometer
Summary
Line-field confocal optical coherence tomography (LC-OCT) is a recently introduced interferometric imaging technique, based on the time-domain version of optical coherence tomography (OCT) [1], with line illumination of the sample and line detection of the signal instead of point scanning and point detection as in conventional OCT [2]. The focus is continuously adjusted during the scan of the sample depth, allowing the use of a high numerical aperture (NA) microscope objective to image with high lateral resolution. A major interest of LC-OCT, as a time-domain OCT (TD-OCT) technique, is the possibility to continuously adjust the focus as a function of depth, since the depth information is acquired sequentially It is one of the most e cient method to optimize the lateral resolution, dynamic focusing in conventional point-scanning TD-OCT is challenging for real-time imaging since both a tracking speed on the order of a few m/s and a repetition rate in the kilohertz range are needed [13,14,15,16]. An image of human skin is shown as an illustration of the performance of the device for biological tissue imaging in vivo
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