Abstract
Background: The decreased level of miR-192-5p has been reported in several kinds of cancers, including bladder, colon, ovarian, and non-small cell lung cancer. However, the expression and function of miR-192-5p in papillary thyroid carcinoma/cancer (PTC) remains unknown.Objective: The present study aimed to explore the function and underlying mechanism of miR-192-5p in PTC development.Methods: PTC tissues and relative normal controls from PTC patients were collected. qRT-PCR analysis was performed to measure miR-192-5p and SH3RF3 mRNA level in PTC tissues and cell lines. CCK-8 method and FCM assay were used to test cell proliferation and apoptosis in TPC-1 cells, respectively. The abilities of cell migration and invasion were detected by wound healing and transwell assays, respectively. The protein expression was evaluated by Western blot. The interaction between miR-192-5p and Src homology 3 (SH3) domain containing ring finger 3 (SH3RF3) were confirmed by dual-luciferase reporter assay.Results: MiR-192-5p level was obviously decreased in PTC tissues and cell lines. Overexpression of miR-192-5p suppressed proliferation, migration, invasion, and EMT process, while induced apoptosis in TPC-1 cells. In addition, miR-192-5p negatively modulated SH3RF3 expression by binding to its 3′-untranslated region (3′UTR). Silencing SH3RF3 inhibited the migration, invasion, and EMT of TPC-1 cells. In the meantime, matrine, an alkaloid extracted from herb, exerted its anti-cancer effects in PTC cells dependent on increase in miR-192-5p expression and decrease in SH3RF3 expression.Conclusion: We firstly declared that miR-192-5p played a tumor suppressive role in PTC via targeting SH3RF3. Moreover, matrine exerted its anti-cancer effects in PTC via regulating miR-192-5p/SH3RF3 pathway.
Highlights
Papillary thyroid carcinoma/cancer (PTC) is the most prevalent subtype of thyroid cancer, occupying more than 75% of all thyroid cancer cases
MiR-192-5p expression was measured in human thyroid follicular epithelial cell line Nthy-ori 3-1 and papillary thyroid carcinoma/cancer (PTC) cell lines BHT101, B-CPAP, and TPC-1
The results showed that miR-192-5p level was decreased dramatically in three PTC cell lines compared with the normal control cell line (Figure 1B)
Summary
Papillary thyroid carcinoma/cancer (PTC) is the most prevalent subtype of thyroid cancer, occupying more than 75% of all thyroid cancer cases. MiRNAs could act as either oncogenes or tumor suppressors by binding to 3 -untranslated region (3 UTR) of their targeted genes, leading to mRNA degradation or transcriptional suppression, regulating tumor development [4,5,6,7]. The expression and function of miR-192-5p in papillary thyroid carcinoma/cancer (PTC) remains unknown. QRT-PCR analysis was performed to measure miR-192-5p and SH3RF3 mRNA level in PTC tissues and cell lines. Overexpression of miR-192-5p suppressed proliferation, migration, invasion, and EMT process, while induced apoptosis in TPC-1 cells. Matrine, an alkaloid extracted from herb, exerted its anti-cancer effects in PTC cells dependent on increase in miR-192-5p expression and decrease in SH3RF3 expression. Matrine exerted its anti-cancer effects in PTC via regulating miR-192-5p/SH3RF3 pathway
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have