Insulin-Like Growth Factor 1: A Promoter of Proliferation and Metastasis in Papillary Thyroid Carcinoma Cells

Abstract

To determine the effect and mechanism of Insulin-like growth factor 1 (IGF-1) on papillary thyroid carcinoma (PTC) cells. We use TPC-1, one of PTC cell lines to evaluate the effects of IGF-1. SiRNA is used to reduce the effect of IGF-1R. In this study, TPC-1 cells were treated with recombinant human IGF-1, and the effects of IGF-1 on proliferation, migration, invasion and apoptosis of TPC-1 cells were studied by using Cell counting kit-8, 5-ethynyl-2′-deoxyuridine, colony formation, Transwell assay and flow cytometry. Compared with the control group, the proliferation ability of TPC-1 cells stimulated by IGF-1 was significantly increased. Cell cycle and apoptosis are not affected by IGF-1. IGF-1 enhances the ability of migration and invasion of TPC-1 cells. The expression of MMP2 increased and the expression of p53 decreased after IGF-1 stimulation in TPC-1 cells. After IGF-1 stimulation, the proliferation, migration and invasion ability of TPC-1 cells were enhanced. MMP2 and p53 may play an important role in proliferation and migration. This may provide a new therapeutic target for patients with PTC.