Mild and efficient enzymatic oximolysis by supported Pseudomonas cepacia lipases

Abstract

Pseudonomas cepacia lipase supported on ceramic particles (lipase PS-C) and diatomite (lipase PS-D) catalyzed the acylation of aldoximes and ketoximes. Aliphatic oximes reacted faster than aromatic oximes and polar solvents enhanced the reaction rate. For lipase PS-C, THF was a superior solvent, while for lipase PS-D 1,4-dioxane was the ideal solvent. The amount of enzyme required to catalyze this reaction was optimized. It was found that for lipase PS-C or lipase PS-D, 50 mg was the optimum amount to catalyze 1.0 mmol of substrate.