Migration increases mitochondrial oxidative capacity without increasing reactive oxygen species emission in a songbird.

Abstract

Birds remodel their flight muscle metabolism prior to migration to meet the physiological demands of migratory flight, including increases in both oxidative capacity and defence against reactive oxygen species. The degree of plasticity mediated by changes in these mitochondrial properties is poorly understood but may be explained by two non-mutually exclusive hypotheses: variation in mitochondrial quantity or individual mitochondrial function. We tested these hypotheses using yellow-rumped warblers (Setophaga coronata), a Nearctic songbird which biannually migrates two to five thousand kilometres. We predicted higher flight muscle mitochondrial abundance and substrate oxidative capacity and decreased reactive oxygen species emission in migratory warblers captured during autumn migration compared to a short-day photoperiod-induced non-migratory phenotype. We assessed mitochondrial abundance via citrate synthase activity and assessed isolated mitochondrial function using high-resolution fluororespirometry. We found 60% higher tissue citrate synthase activity in the migratory phenotype, indicating higher mitochondrial abundance. We also found 70% higher state 3 respiration (expressed per unit citrate synthase) in mitochondria from migratory warblers when oxidizing palmitoyl-carnitine, but similar H2O2 emission rates between phenotypes. By contrast, non-phosphorylating respiration was higher and H2O2 emission rates were lower in the migratory phenotype. However, flux through electron transport system complexes I-IV, II-IV and IV were similar between phenotypes. In support of our hypotheses, these data suggest that flight muscle mitochondrial abundance and function are seasonally remodeled in migratory songbirds to increase tissue oxidative capacity without increasing reactive oxygen species formation.