Abstract
Delignification from the cell walls with ozone using thin sections of a softwood, Japanese cypress (Chamaecyparis obtusa Endl.) was examined by UV- and visible-light (VL) microspectrometry coupled with the Wiesner reaction. The VL- and UV-absorption spectra revealed that delignification from the cell walls with ozone increases with increasing ozonization time and proceeds from the lumen toward the middle lamella within the secondary wall of a cell. The secondary wall of tracheids were ozonized faster than the middle lamella of cell corners. This may be due to a difference in the lignin content between both tissues. In addition, there might be a difference in the longitudinal and transverse permeabilities of ozone within the secondary wall of tracheids. The results obtained here revealed that VL-microspectrometry coupled with the Wiesner reaction is useful for the analysis of lignin content in the cell walls.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call