Abstract
Aberrant expression of microRNAs (miRs) has been reported in various types of cancer. The aim of the present study was to investigate the role and underlying molecular mechanism of miR‑130a‑3p in cervical cancer (CC). The expression of miR‑130a‑3p in CC tissues and cell lines (CaSki and SiHa) was measured via reverse transcription‑quantitative PCR. SiHa and CaSki cells were transfected with miR‑130a‑3p mimics and a miR‑130a‑3p inhibitor, respectively. The proliferation, apoptosis and migration and invasion abilities of CC cells were then measured using MTT, flow cytometry, wound‑healing and Transwell assays, respectively. TargetScan and dual‑luciferase reporter gene assays were performed to analyze the association between miR‑130a‑3p and its predicted target gene Runt‑related transcription factor 3 (RUNX3). In addition, a xenograft tumor model was established in mice to evaluate the impact of miR‑130a‑3p on tumor growth invivo. The expression of miR‑130a‑3p was markedly upregulated in CC tissues and cell lines compared with normal tissues and cells. Transfection with miR‑130a‑3p mimics significantly promoted the proliferation, migration and invasion, and inhibited the apoptosis of SiHa cells. Treatment of CaSki cells with a miR‑130a‑3p inhibitor resulted in opposite effects to those of miR‑130a‑3p mimics. RUNX3 was identified as the target gene of miR‑130a‑3p, and overexpression of RUNX3 eliminated the tumor‑promoting effect of miR‑130a‑3p mimics on CC cells. Overexpression of miR‑130a‑3p also promoted tumor growth in mice. In conclusion, miR‑130a‑3p promoted proliferation, migration and invasion, and inhibited the apoptosis of CC cells via targeting RUNX3, suggesting a novel treatment target for CC.
Highlights
Cervical cancer (CC) frequently occurs in women over the age of 15 years and is the second leading cause of cancer‐ associated mortality [1]
MiR‐149 has been suggested to inhibit the proliferation and promote the apoptosis of CC cells via targeting GIT1 [10]. miR‐130a has emerged as an important miRNA in the development and progression of numerous types of malignancy, including hepatocellular carcinoma (HCC), ovarian cancer, glioblastoma, prostate carcinoma and CC [11]
The proliferation detected via MTT analysis was significantly increased in the miR‐130a‐3p mimics group compared with that in the mimics‐NC group (P
Summary
Cervical cancer (CC) frequently occurs in women over the age of 15 years and is the second leading cause of cancer‐ associated mortality [1]. The treatments for patients with CC primarily include surgery, radiotherapy and chemotherapy [4]. Despite their wide application in clinical practice, the outcome remains unsatisfactory, which is mainly due to the metastasis of CC [5]. MiR‐130a has emerged as an important miRNA in the development and progression of numerous types of malignancy, including hepatocellular carcinoma (HCC), ovarian cancer, glioblastoma, prostate carcinoma and CC [11]. Liu et al [13] further suggested that upregulation of miR‐130a‐3p expression suppressed the migration and invasion of HCC cells through downregulating expression of its target gene SMAD4. The biological function of miR‐130a‐3p in CC remains unclear
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