Abstract
The purpose of the present study is to figure out the role of miRNA-148a (miR-148a) in growth, apoptosis, invasion, and migration of cervical cancer cells by binding to regulator of ribosome synthesis 1 (RRS1). Cervical cancer and adjacent normal tissues, as well as cervical cancer cell line Caski, HeLa, C-33A, and normal cervical epithelial cell line H8 were obtained to detect the expression of miR-148a and RRS1. Relationship between miR-148a and RRS1 expression with clinicopathological characteristics was assessed. The selected Caski and HeLa cells were then transfected with miR-148a mimics, miR-148a inhibitors or RRS1 siRNA to investigate the role of miR-148a and RRS1 on proliferation, apoptosis, colony formation, invasion, and migration abilities of cervical cancer cells. Bioinformatics information and dual luciferase reporter gene assay was for used to detect the targetting relationship between miR-148a and RRS1. Down-regulated miR-148a and up-regulated RRS1 were found in cervical cancer tissues and cells. Down-regulated miR-148a and up-regulated RRS1 are closely related with prognostic factors of cervical cancer. RRS1 was determined as a target gene of miR-148a and miR-148a inhibited RRS1 expression in cervical cancer cells. Up-regulation of miR-148a inhibited cell proliferation, migration, and invasion while promoting apoptosis in Caski and HeLa cells. Our study suggests that miR-148a down-regulates RRS1 expression, thereby inhibiting the proliferation, migration, and invasion while promoting cell apoptosis of cervical cancer cells.
Highlights
Cervical cancer is known as the third most common cancer and the fourth leading reason of cancer-related death in female in the world [1]
Down-regulated miR-148a and up-regulated regulator of ribosome synthesis 1 (RRS1) are found in cervical cancer tissues
The results suggested that down-regulated miR-148a and up-regulated RRS1 are closely related with prognostic factors of cervical cancer
Summary
Cervical cancer is known as the third most common cancer and the fourth leading reason of cancer-related death in female in the world [1]. Statistics show that more than 50,000 females succumb to cervical cancer each year in China [2]. Great advances have been achieved in surgery, irradiation, as well as chemotherapy for the treatment of cervical cancer, but the prognosis of patients with cervical cancer remains to be unsatisfactory owing to late diagnosis [5, 6]. The specific molecular mechanisms referring to the initiation and progression of cervical cancer are still unknown. Better understanding the mechanisms for the occurrence and progression of cervical cancer will help to seek for the novel biomarkers and treatment targets, all of which is pivotal for improving the prognosis of patients with cervical cancer
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