Abstract
Rubus brasiliensis and Rubus erythroclados are commonly known as greenberries. This work aimed to test in vitro micropropagation protocols to greenberries. The multiplication experiment was conducted using BAP at concentrations 1 mg L-1, 2 mg L-1 and 3 mg L-1. The rooting experiment was carried out in ½ MS medium without the addition of hormones, using 0.1 mg L-1 NAA or 1.0 mg L-1 IBA. The highest rate of viability of explants was obtained with R. brasiliensis propagation using 1.0 mg L-1 of BAP. Rooting explants showed survival rates higher than 85%. Rooting rates in R. erythroclados was 87% while in R. brasiliensis it was 38%, The highest rates were obtained using 1.0 mg L-1 IBA in R. erythroclados.The acclimatization reached survival rates of 75% when rooted using NAA and ½ MS and 86% when rooted with IBA. In vitro culture shows to be a viable method to propagate greenberries.
Highlights
In Brazil, 11 species of the genus Rubus are described, six of these occur in the Santa Catarina state and are associated with the Atlantic Forest Biome (Reflora, 2019; Reitz, 1996)
The objective of this work was to define an in vitro micropropagation protocol for greenberries in order to contribute to the domestication of R. brasiliensis and R. erythroclados
The vegetative material used in this study came from parent plants of Rubus brasiliensis and R. erythroclados kept in a greenhouse (26 ± 2° C) with intermittent irrigation (Figure 1A)
Summary
In Brazil, 11 species of the genus Rubus are described, six of these occur in the Santa Catarina state and are associated with the Atlantic Forest Biome (Reflora, 2019; Reitz, 1996). Around 500 ha of blackberries originating from genetic material native from United States are cultivated in the south and southeast of Brazil (Antunes, et al, 2014). This fruit consumption is associated with nutraceutical properties, such as anticarcinogenic compounds (Magalhães, Maciel & Orsolin, 2017), which are present in greenberries
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