Abstract
An efficient protocol for in vitro clonal propagation of A. mangium was developed using seedling derived explants. Out of three different explants tested for shoot proliferation, cotyledonary node showed best performance than leaf node and shoot tip explants. MS (Murashige and Skoog’s) medium was found best for shoot proliferation and cotyledonary nodes were subsequently cultured on MS medium supplemented with BA and Kn alone or in combination with NAA, IBA and GA 3 at different concentrations. Maximum number of shoots was formed on MS medium containing 4.0 µM BA. For adventitious rooting, in vitro proliferated shoots were transferred to full strength MS medium fortified with IBA and NAA singly at different concentrations (0-8.0 µM). Best rooting responses were observed in the medium containing 8.0 µM IBA. Plantlets having well developed root system were transferred to soil and successfully acclimatized with 65% survival rate under ex vitro condition.
Highlights
A. mangium Willd., belonging to Mimosaceae family, is one of the most important leguminous tree characterized by its fast growth, nitrogen fixing ability, good growth in adverse soil condition and a tendency to grow well in humid and hot climate (Umezawa et al, 2008)
Similar results were noted in a range of legume species, such as A. senegal (Khalafalla and Daffalla, 2008), A. sinuata (Vengadesan et al, 2002b), Clitoria ternatea (Barik et al, 2007), Colutea istria (Hegazi and Gabar, 2010), Dalbergia sissoo (Pradhan et al, 1998a), D. latifolia (Pradhan et al, 1998b), Pterocarpus marsupium (Anis et al, 2005), P. santalinus (Rajeswari and Paliwal, 2008) and Peltophorum pterocarpum (Uddin et al, 2005)
This study revealed that full strength MS medium was preferred for axillary shoot proliferation from cotyledonary nodes of A. mangium while WPM showed a little effect in terms of shoot proliferation
Summary
A. mangium Willd., belonging to Mimosaceae family, is one of the most important leguminous tree characterized by its fast growth, nitrogen fixing ability, good growth in adverse soil condition and a tendency to grow well in humid and hot climate (Umezawa et al, 2008). It is native to northern Queensland of Australia, through Papua New Guinea into the Indonesian provinces of Irian Jaya and Maluku This tree species is widely used for timber, pulp and fire wood (Galiana et al, 1991). There are a few successful reports on in vitro clonal propagation of A. mangium using seedling derived explants like seedling nodes (Ahmad, 1991; Galiana et al, 1991; Saito et al, 1993), but no noted reports on its clonal propagation employing cotyledonary node explants have been found yet This communication, describes a successful and quick method on large scale in vitro clonal propagation of A. mangium via cotyledonary node explants
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