Abstract
This research was carried out at the Plant Tissue Culture Laboratory (PTCL) in Faculty of Environmental Agricultural Sciences, Arish University, Egypt during 2013 to 2016. The aim of this study was using tissue culture technique for the micropropagation of Origanum syriacum L. plant. One node cutting and shoot tip were cultured on Murashige and Skoog (MS), Nitsch and Nitsch (NN) and Gamborg (B5) media. The results showed that shoot tip cultured on the MS medium had the best combination for the establishment stage of mother plants. Shoots cultured on MS medium supplemented with0.50 mgl-1 Kin and 0.05 mgl-1NAA produced the highest shoots developed during multiplication stage. MS medium supplemented with 0.40 mgl-1 thiamine achieved the best shoots development compared with the other additive treatments and control. Addition of 1 mgl-1IBA to the full strength MS medium and 1.00 mgl-1 IAA to the half strength MS had the highest number of main roots and root lengths. Hardening the produced rooted plantlets were done in a greenhouse in pots containing mixture of peatmoss, vermiculite and washed sand at the rate (1:1:1) and after adjustment of mixture pH. Plantlets were successfully acclimated with 93 % survive.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
