Abstract
A prolific plant regeneration system using scalps derived from shoot tips of Musaspp. cv. Tanduk was developed. Highly proliferating scalps, produced after four monthly subcultures of shoot tip explant on Murashige and Skoog (MS) medium supplemented with 100 mM BAP and 1.0 mM IAA, were placed on MS basal medium supplemented with 1.0, 2.5 and 5.0 mM BAP. Rooting of shoots was assessed on hormone-free half strength and full strength MS media and on MS medium supplemented with 1.0, 5.0 and 10 mM IBA. Four types of potting media comprising of sand, peat, sand + top soil + goat dung (3:2:1 v/v) and top soil + sand (1:1 v/v) were evaluated during acclimatization of the plantlets. Prolific shoot regeneration from scalps was obtained on MS medium containing 2.5 mM BAP, at 9.61 and 40.6 shoots per explant after 4 and 8 weeks of culture, respectively. Meanwhile, the highest mean shoot height of 2.19 cm was attained on MS medium with 1.0 mM BAP after 8 weeks of culture. Full-strength MS medium supplemented with 5.0 mM IBA produced the highest mean number of roots per explant at 15.08, while the highest mean root length of 11.07 cm was obtained on hormone-free half strength MS medium at week 4 of culture. The highest plant survivability of 77.5% was achieved in potting medium consisting of top soil + sand + goat dung after 6 weeks of acclimatization. The plants were morphologically normal with vigorous stems and broad green leaves. Key words: In vitro, shoot tip, scalps, regeneration.
Highlights
Bananas and plantains (Musa sp.) are perennial monocotyledonous giant herbaceous plants grown in many tropical areas
This paper describes prolific shoot regeneration from scalps of Musa sp. cv
Through selection of the right meristematic clumps, along with monthly subculture, good quality scalps (Figure 1A) were obtained on Murashige and Skoog (MS) medium supplemented with 100 μM benzyl amino purine (BAP) and 1.0 M indole-3-acetic acid (IAA) after 4 months
Summary
Bananas and plantains (Musa sp.) are perennial monocotyledonous giant herbaceous plants grown in many tropical areas. The fruits are used both as staple food (cooking banana) and dietary supplement (dessert banana) (Assani et al, 2001). The total world production of banana reached 70.8 million metric tonnes in the year 2006 (FAO, 2007). Tanduk is a cooking banana, having the largest fruit among the banana cultivars, with a light creamy orange pulp, fine in texture but firm. The fruit has Abbreviations: BAP, benzyl amino purine; IAA, indole-3-acetic acid; IBA, indole-3-butyric acid; MS, Murashige and Skoog (1962) medium
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