Microplastics alter the microbiota-mediated phosphorus profiles at sediment-water interface: Distinct microbial effects between sediment and plastisphere

Abstract

Microplastics (MPs) are ubiquitous in freshwater sediments, raising concern about their potential impacts on ecosystem services. However, the specific impacts of microbiota mediated by MPs in sediment and plastisphere compartments on P availability remain elusive. This investigation conducted a series of microcosm experiments utilizing eutrophic lake sediment amended with fuel-based polyethylene terephthalate (PET), bio-based polylactic acid (PLA) MPs, and a natural cobblestone substrate to unravel their effects. The findings highlighted that MPs induced alterations in bacterial communities in both sediment and plastisphere, consequently modifying P availabilities at the sediment-water interface (SWI). In comparison to non-biodegradable PET, biodegradable PLA MPs presented higher proportions of specific bacteria and functional genes associated with P profiles, such as Firmicutes, Ignavibacteriota, and P mineralizing genes in the sediment and plastisphere. This, in turn, elevated the levels of soluble reactive P in the porewater by 54.19 % (0–1 cm), 55.81 % (1–3 cm), and 18.24 % (3–5 cm), respectively. Additionally, PLA obviously altered P immobilization capacity and bioavailability, increasing the organic P fraction. Whereas, inert cobblestone exhibited negligible influence on P biogeochemical processes during the incubation. Moreover, the biofilm communities and those in the surrounding sediment specifically contributed to the changes in P profiles at the SWI. The functional genes associated with P profiles in the sediment mainly concentrate on P mineralization and P uptake/transport. In the plastisphere, P activation genes are obviously affected under MP exposure. This study fills the knowledge gap concerning the repercussions of MPs on ecosystem services.