Abstract
This paper describes the first demonstration of the combination of micropatterned paper devices (μPPDs) and polydiacetylene (PDA) colorimetric probes for double-stranded DNA (dsDNA) detection with high sensitivity and fast response. The device is rapidly fabricated on paper by wax-screen-printing. The amine-functionalized PDA vesicles are spotted to the paper chambers, followed by the introduction of the polymerase chain reaction (PCR)-amplified dsDNA targets. After the completion of the ionic interactions between the targets and the probes, a significantly different optical signal is produced, thereby facilitating the naked-eye detection. Additionally, the signals are readily documented through a smartphone for quantitative analysis. In this study, the PDA colorimetric assay in μPPDs is capable of discriminating the presence of the target down to 10nM (the typical concentration of PCR amplicons is around 100nM). At such concentration, the value of colorimetric response in paper is about 41% higher than that in tube, leading to an enhanced sensitivity. In addition, the colorimetric reaction in μPPDs is accomplished in 1min, and the analytical speed is much faster than that observed in tube. These results show that the paper-based PDA colorimetry should have greatly enhanced applications as a technology for DNA diagnostics.
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