Abstract
Microbial community studies in treatment wetlands (TW) have not investigated cold-climate TW and have focused primarily on laboratory mesocosms. This study determined microbial community composition and dynamics using 16s rRNA sequencing in a partially-saturated two-stage, vertical-flow pilot TW treating ski resort wastewater from December–April. Indicator species analysis found organisms capable of the primary nitrogen transformations observed in each stage at 3 °C, with nitrifiers in the unsaturated second stage and heterotrophic denitrifiers in the first stage where recycled nitrate is removed. During seasonal TW operation, microbial communities developed significant differences between TW stages and α-diversity decreased. Microbial communities were compared to batch mesocosm TW communities, which were significantly different from the pilot. The study is the first to discern community dynamics and key microorganisms during seasonal loading of a cold-climate, field-scale TW. Results raise questions regarding comparability of mesocosms to pilot systems and indicate more field-scale TW investigations are needed.
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