Metabolic Profiling by Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) and Electrospray Ionization Quadrupole Time-Of-Flight Mass Spectrometry (ESI-Q-TOF-MS)

Kazuo Hirayama

doi:10.1007/4-431-28055-3_6

Metabolic Profiling by Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) and Electrospray Ionization Quadrupole Time-Of-Flight Mass Spectrometry (ESI-Q-TOF-MS)

Kazuo Hirayama

https://doi.org/10.1007/4-431-28055-3_6

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Publication Date: Jan 1, 2005

Citations: 1

Affiliation: Ajinomoto (Japan)

#Electrospray Ionization Quadrupole Time-Of-Flight Mass Spectrometry #Fourier-transform Ion Cyclotron Resonance Mass Spectrometry + Show 8 more

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Abstract

Metabolic profiling by FT-ICR-MS and ESI-Q-TOF-MS is an effective method to capture a rough image of metabolism. It should be performed after the accuracy is confirmed by using the authentic sample after an elaborate calibration is done, when an individual metabolite is identified from the accurate mass for FT-ICR-MS. Moreover, when ESI-Q-TOF-MS is used, the CID spectrum should also be measured, and compared with that of the authentic sample.

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