Mechanically Activated Enzymatic Hydrolysis of Yeast Biomass

Abstract

Experimental studies were focused on the processes that occur during mechanoenzymatic hydrolysis of cell wall polymers of yeast Saccharomyces cerevisiae. Particular attention was paid to the reactivities of polymers involved in the supramolecular structures of the cell walls, as well as to the technology scaling-up using pilot and industrial facilities. The Russian commercially available enzymatic agent CelloLux 2000 (from Sibbiofarm LLC, Berdsk) was used for the studies. It was shown that mechanical treatment of the yeast biomass allows the supramolecular structure of cell wall polymers to be changed and their reactivity to enzymatic hydrolysis to be improved. The yields of mannanoligosaccharide (1,1 %) and mannanoproteins (2,7 %) to the alkali extract were determined. A phenomenological model based on autolocalized process mechanism was suggested for the mechanically activated enzymatic hydrolysis. Pilot and industrial facilities of Sibbiofarm LLC was used for the technology scaling-up and for preparation of the new mannanoligosaccharide product for animal industry. A pilot batch of the product was tested, its efficiency in fighting salmonellosis and gaining in weight of animals demonstrated.