Mechanically activated enzymatic hydrolysis of yeast biomass

Abstract

Experimental data obtained in studying processes that occur during the mechanoenzymatic hydrolysis of cell wall polymers of Saccharomyces cerevisiae yeast are presented. Special emphasis is placed on studying the reactivity of polymers contained in the supramolecular structure of cell walls, and on scaling the technology using pilot and industrial equipment. It is shown that mechanical treatment of yeast biomass allows us to alter the supramolecular structure of cell wall polymers and increase their reactivity in the enzymatic hydrolysis. Mechanical treatment disintegrates yeast cells and changes the supramolecular structure of polymers in their cell walls; the structural layers of a cell wall become disordered and diffused. A phenomenological model of mechanically activated enzymatic hydrolysis is proposed; the model is based on an autolocalization mechanism of the process. The domestic commercially available enzyme preparation CelloLux 2000 (OOO PA Sibbiopharm, Berdsk, Novosibirsk oblast) is used in this work. It is shown that changes in the supramolecular structure of yeast cell wall polymers occur during mechanically activated enzymatic hydrolysis. The yields of mannanoligosaccharides (1.1%) and mannanoproteins (2.7%) in the main extract are determined. Using pilot and industrial equipment at OOO PA Sibbiopharm, the technology is scaled and the production of a new mannanoligosaccharide product for animal husbandry is established. The pilot batch of the final product is effective against salmonellosis and is shown to increase the weight gain of experimental animals.