Management of DNA Information Bank Using Typifix Method for Microsatellite Analysis and Snp Assays in Order to Ensure Safety Foods By Animal Filiations and Traceability for Farm Animal Biodiversity Under the New Circumstances of the Eco-Economy Paradigm

Abstract

Molecular biology tests based on identification, amplification and characterization of nucleic acid revolutionized the pathology diagnostic, conservation of indigene animal genetic resources, gene assisted selection and food traceability from farm-to-fork and from fork-to-farm. The Deoxyribonucleic Acid (DNA) of each animal is different (with the exceptions of monozygotic twins and clones). Since the genome of each animal contains approximately three billion DNA units, the range for variation among the DNA sequences of animals is enormous, consequently DNA markers analysis allows assuring a traceability of 100% in the meat industry. A methodology using 17 ISAG (International Society for Animal Genetics) DNA microsatellite markers is proposed for meat traceability. The original methods - patented by Prof.Brem and his Agrobiogen team - that were used in Romania to reveal DNA polymorphism are described as their applicability in species identification and meat traceability. The objective it was analysis the DNA profile of pig with 10 microsatellite markers for meat identity control, of sheep for scrapie diagnostic and cattle for performants selection .