Abstract
Measurements of lysozyme diffusion within the “depletion zone” of the (1 1 0) face of lysozyme crystals are reported. The measurements were performed using the technique of microscope light scattering , capable of measuring small sampling areas (∼2 μm 2). Measurements within such small areas allowed for the determination of intensity correlation functions at precise locations (at ∼10 μm intervals) adjacent to the (1 1 0) crystal face. From the intensity correlation functions, diffusion coefficients of lysozyme molecules adjacent to the (1 1 0) crystal face and in solution were determined. The diffusion coefficients were observed to decrease as the surface of the crystal was approached.
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