Abstract
1. 1. High concentrations of Mg 2+ in the culture medium inhibit lysis of ØX174-infected Escherichia coli C bacteria, thus yielding a 5–10-fold higher phage production. 90% or more of the phages produced under these conditions can be sedimented together with the infected cells. The phages are quantitatively extracted from the bacterial sediment after lysis induced by EDTA. 2. 2. A similar inhibitory effect of Mg 2+ on the lysis of M12-infected E. coli K12 AB and Qβ-infected Q13 cells, resulting in a 2–3-fold higher phage yield, is reported.
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