Abstract
Ultraviolet (UV) irradiation induces significant changes to skin connective tissues as a result of the degradation of collagen, which is a major structural component of the extracellular matrix. This process may be mediated by matrix metalloproteinases (MMPs). In this study, we examined the protective effect of a polyphenolic flavone, luteolin, on the expression of two matrix metalloproteinases, MMP-1 and MMP-9, in UVA- and UVB-irradiated human dermal fibroblast cells. Luteolin is found in many medicinal plants as well as in a large number of vegetables, fruits and a variety of spices. It has a number of biological activities including anti-cancer, anti-oxidant, anti-inflammatory, antiallergic and immunomodulatory activities. Human dermal fibroblast (HDF) cells were treated with luteolin at 1-10 μM, then irradiated with UVA at 10 J/cm2 and UVB at 200 mJ/cm2. Cells and culture supernatant were harvested 24 h after irradiation. Our results show that luteolin at 1-10 μM dose-dependently suppressed the expression of MMP-1 and MMP-9 genes in UVA and UVB-exposed HDF cells, as measured by quantitative real-time reverse transcription–polymerase chain reaction (qRT-PCR). Luteolin was also found to reduce the production of MMP-1 protein in UVA and UVBexposed HDF cells detected by enzyme-linked immunosorbent assay (ELISA) in a dose-dependent manner. The release of MMP-9 was also reduced by luteolin in UVB-irradiated HDF cells in a dose-dependent manner. Our results indicate that luteolin can inhibit UV-induced MMP-1 and MMP-9 expression in HDF cells. Therefore, they may be potentially useful in the prevention and treatment of skin photoaging.
Highlights
Ultraviolet irradiation causes distinct changes to skin collagenous tissues as a result of the breakdown of collagen, a major component of the extracellular matrix
It was suggested that excessive matrix degradation by UVinduced matrix metalloproteinases (MMPs)-1 and MMP-9 secreted by keratinocytes and fibroblast cells contributes substantially to connective tissue damage that occurs during photo aging [8,9]
The results show that UVA and UVB induced the production of MMP-1 as well as MMP-9 in Human dermal fibroblast (HDF) cells
Summary
Ultraviolet irradiation causes distinct changes to skin collagenous tissues as a result of the breakdown of collagen, a major component of the extracellular matrix. ROS-induced molecular damage produces a number of harmful effects on cellular function and homeostasis, while degradation of extracellular matrix (ECM) proteins, such as collagen, by ROS can cause major changes in skin connective tissue [1,2,3,4,5]. It was suggested that excessive matrix degradation by UVinduced MMP-1 and MMP-9 secreted by keratinocytes and fibroblast cells contributes substantially to connective tissue damage that occurs during photo aging [8,9]. This evidence suggests that the expression of MMP-1 as well as MMP-9 plays a major role in the process of photoaging in fibroblast cells
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.