Abstract
ObjectivesTo investigate the effect of lupeol in a mouse model of viral myocarditis induced by coxsackie virus B3 (CVB3).MethodsMice were separated into controls (DMEM, n = 20) and CVB3 infected groups (i.e., untreated CVB3 [n = 40]; CVB3 + lupeol 50 mg/kg [n = 40]; CVB3 + lupeol 100 mg/kg [n = 40]; CVB3 + small interfering RNA (siRNA)- toll-like receptor 4 (TLR4) [n = 20]; siRNA + EXP-H mice [n = 20]). Reverse transcription polymerase chain reaction (RT-PCR), western-blot assay, immunohistochemistry, enzyme-linked immunosorbent (ELISA) assay and histopathology were performed to investigate the cardioprotective role of lupeol.ResultsThe elevated pro-inflammatory cytokines in CVB3-infected mice (i.e., interleukin-1β [IL-1β]; interleukin-6 [IL-6]; tumour necrosis factor-α [TNF-α]) were significantly reduced by lupeol 50 or 100 mg/kg. Interestingly, the mRNA level and protein level of toll-like receptor 4 (TLR4) were inhibited by lupeol.ConclusionsLupeol alleviates CVB3-induced viral myocarditis and myocardial damage in mice. The underlying mechanism may due to downregulation of TLR4.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
